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首页> 外文OA文献 >Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain.
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Cloning of three human tyrosine phosphatases reveals a multigene family of receptor-linked protein-tyrosine-phosphatases expressed in brain.

机译：三种人类酪氨酸磷酸酶的克隆揭示了在大脑中表达的受体连锁蛋白酪氨酸磷酸酶的多基因家族。

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摘要

A human brainstem cDNA library in bacteriophage lambda gt11 was screened under conditions of reduced hybridization stringency with a leukocyte common antigen (LCA) probe that spanned both conserved cytoplasmic domains. cDNA encoding a receptor-linked protein-tyrosine-phosphatase (protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48), RPTPase alpha, has been cloned and sequenced. Human RPTPase alpha consists of 802 amino acids. The extracellular domain of 150 residues includes a hydrophobic signal peptide and eight potential N-glycosylation sites. This is followed by a transmembrane region and two tandemly repeated conserved domains characteristic of all RPTPases identified thus far. The gene for RPTPase alpha has been localized to human chromosome region 20pter-20q12 by analysis of its segregation pattern in rodent-human somatic cell hybrids. Northern blot analysis revealed the presence of two major transcripts of 4.3 and 6.3 kilobases. In addition to RPTPase alpha, two other RPTPases (beta and gamma), identified in the same screen, have been partially cloned and sequenced. Analysis of sequence comparisons among LCA, the LCA-related protein LAR, and RPTPases alpha, beta, and gamma reveals the existence of a multigene family encoding different RPTPases, each containing a distinct extracellular domain, a single hydrophobic transmembrane region, and two tandemly repeated conserved cytoplasmic domains.
机译：在具有降低的杂交严格性的条件下，与跨越两个保守的胞质结构域的白细胞共同抗原（LCA）探针，筛选了噬菌体λgt11中的人脑干cDNA文库。已经克隆并测序了编码受体连接的蛋白质酪氨酸磷酸酶（蛋白质酪氨酸磷酸磷酸水解酶，EC 3.1.3.48）的cDNA。人RPTPaseα由802个氨基酸组成。 150个残基的胞外域包括一个疏水信号肽和八个潜在的N-糖基化位点。紧随其后的是跨膜区和迄今为止鉴定出的所有RPTPase的两个串联重复的保守结构域。通过分析其在啮齿动物-人类体细胞杂种中的分离模式，RPTPaseα的基因已定位于人类染色体区域20pter-20q12。 Northern印迹分析表明存在两个主要的转录本，分别为4.3和6.3千碱基。除了RPTPaseα外，在同一筛选中鉴定出的另外两个RPTPase（β和γ）已被部分克隆和测序。 LCA，LCA相关蛋白LAR和RPTPasesα，β和gamma之间的序列比较分析显示，存在一个编码不同RPTPase的多基因家族，每个家族包含一个不同的胞外域，一个疏水跨膜区和两个串联重复保守的胞质结构域。

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